Products /
Secondary Antibodies /
Anti-mouse IgG (H+L) (DyLight 680 Conjugate)

Anti-mouse IgG (H+L) (DyLight 680 Conjugate) #5470

Filter:

Supporting Data

REACTIVITY	M
SENSITIVITY
MW (kDa)
Source/Isotype	Goat

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

Anti-mouse IgG (H+L) was conjugated to DyLight 680 fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 684 nm and peak fluorescence emission is 715 nm.

Product Usage Information

The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications.

Fluorescent western blotting: 1:15000

In-Cell Western: 1:1000

Storage

Supplied in 100 mM PBS, pH 7.2, containing 1% BSA and 0.02% sodium azide. Store at 4°C. Protect from Light. Do not freeze.

Protocol

Specificity / Sensitivity

Anti-mouse IgG (H+L) (DyLight 680 Conjugate) reacts with heavy and light chain of most mouse immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species.

Species Reactivity:

Mouse

Source / Purification

This antibody is prepared from goat antibodies and purified by immunoaffinity chromatography using antigen coupled to agarose beads.

Background

Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
This product has been optimized for use as a secondary antibody in fluorescent western blotting and In-Cell Western™.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.