Anti-mouse IgG (H+L), F(ab')2 Fragment (DyLight® 488 Conjugate) #4316
We recommend the following alternatives
Inquiry Info. # 4316
Please see our recommended alternatives.
Supporting Data
| REACTIVITY | |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Goat |
Product Information
Product Description
Product Usage Information
For flow cytometry, use product at a 1:1000 dilution. For immunofluorescence, the optimal dilution may have to be determined. For the detection of many antibodies, the optimal dilution is 1:250-1:1000. See protocol for more details.
Storage
Specificity / Sensitivity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or human serum proteins.
Source / Purification
The Anti-mouse IgG (H+L), F(ab')2 Fragment is produced by immunizing goats with mouse IgG whole molecules. The Anti-mouse IgG (H+L),
F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion and chromatographic separation. The F(ab')2 Fragments were conjugated to DyLight® 488 under optimal conditions with an F/P ratio of 2-6.
Background
Fluorescent Anti-mouse IgG (H+L), F(ab')2 Fragment is ideal for flow cytometry and immunofluorescence. This secondary antibody will detect mouse antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.