Render Target: STATIC
Render Timestamp: 2024-11-22T10:44:09.535Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:17:42.870
Product last modified at: 2024-10-23T17:45:09.019Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7
  1. Products /
  2. Secondary Antibodies /
  3. Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose® Bead Conjugate)

Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose® Bead Conjugate) #5946

Filter:
  • IP

    Supporting Data

    REACTIVITY M
    SENSITIVITY
    MW (kDa)
    Source/Isotype Rabbit
    Application Key:
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in mice.

    Product Usage Information

    Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG Fc, or human serum proteins.

    Species Reactivity:

    Mouse

    Source / Purification

    The Anti-mouse IgG (H+L), F(ab')2 Fragment is produced by immunizing goats with mouse IgG whole molecules. The Anti-mouse IgG (H+L), F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion, and chromatographic separation.

    Background

    Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose Bead Conjugate) is ideal for immunoprecipitation. This secondary antibody will detect mouse antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.

      Limited Uses

      Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

      Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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