Render Target: STATIC
Render Timestamp: 2024-12-20T11:47:42.101Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-07-19 19:14:09.907
Product last modified at: 2024-05-30T07:09:10.692Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Goat Anti-Mouse IgG1 Antibody (AP Conjugate) #48253

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    Affinity purified Goat Anti-Mouse IgG1 antibody is conjugated to alkaline phosphatase (AP). This product has been optimized for use as a secondary antibody in Western blotting applications.

    Product Usage Information

    Recommended Antibody Dilutions:

    1:2000 - 1:8000

    Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.

    Storage

    Supplied in 50 mM Tris, 1 mM MgCl2, pH 8.0, and 50% glycerol containing < 0.1% sodium azide. Store at -20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Goat Anti-Mouse IgG1 Antibody (AP Conjugate) detects the heavy chain of mouse IgG1. This antibody has been cross-adsorbed against mouse IgG2a, IgG2b, IgG3, IgM, and IgA, human, bovine, and horse serum proteins. It may cross-react with immunoglobulins from other species.

    Species Reactivity:

    Mouse

    Source / Purification

    Goat Anti-Mouse IgG1 Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG1. The Anti-Mouse IgG1 antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

    Background

    The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
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