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Goat Anti-Mouse IgM, F(ab')2 Antibody (Low Endotoxin, Azide-free)

Goat Anti-Mouse IgM, F(ab')2 Antibody (Low Endotoxin, Azide-free) #46315

Supporting Data

REACTIVITY	M
SENSITIVITY
MW (kDa)
Source/Isotype	Goat

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

B cells become activated when the B cell Receptor (BCR) interacts with its cognate antigen, resulting in activation of signal transduction cascades that promote proliferation, survival, and differentiation into antibody secreting plasma cells. BCR signaling can be interrogated experimentally through the use of antibodies that crosslink the BCR, which induces phosphorylation of numerous intracellular signaling molecules. These molecular events can be quantified at the single-cell level using applications such as flow cytometry and western blotting (1-3).

Endotoxin

Less than or equal to 0.05 EU/μg, as determined by the LaL assay.

Product Usage Information

Working concentrations and length of treatment can vary depending on the desired effect.

Formulation

Supplied in phosphate buffered saline (PBS), pH7.4, Low Endotoxin, Azide-free.

Storage

Store at -20°C. Aliquot to avoid multiple freeze/thaw cycles.

Specificity / Sensitivity

Goat Anti-Mouse IgM, F(ab')₂ Antibody (Low Endotoxin, Azide-free) detects the heavy chain of mouse IgM and has been cross-adsorbed against mouse IgG, IgA, human immunoglobulins, and pooled sera. This antibody may cross-react with immunoglobulins from other species.

Species Reactivity:

Mouse

Source / Purification

Goat Anti-Mouse IgM, F(ab')₂ Antibody (Low Endotoxin, Azide-free) is produced by immunizing goats with mouse IgM, purified by affinity chromatography on mouse IgM covalently linked to agarose, and pepsin digested.

Background

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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