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Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127

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Supporting Data

REACTIVITY	Rab
SENSITIVITY
MW (kDa)
Source/Isotype	Mouse

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

Rab-Rabbit

Product Information

Product Description

Affinity purified mouse anti-rabbit IgG (Conformation Specific) HRP Conjugate antibody.

Product Usage Information

Recommended Antibody Dilution:

1:2000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. This antibody may cross react with denatured IgG at high concentrations.

Species Reactivity:

Rabbit

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

Background

Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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