Render Target: STATIC
Render Timestamp: 2024-12-27T11:59:42.685Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:16:24.776
Product last modified at: 2024-09-20T07:05:05.354Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677

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Inquiry Info. # 3677

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    Supporting Data

    REACTIVITY
    SENSITIVITY
    MW (kDa) 25
    Source/Isotype Mouse
    Application Key:
    • WB-Western Blotting 

    Product Information

    Product Description

    Affinity purified mouse anti-rabbit IgG (Light-Chain Specific) antibody. This product has been optimized for use as a secondary antibody in Western blotting applications.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb recognizes the light chain of rabbit IgG, either in native conformation or denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

    Background

    Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb as a secondary antibody will eliminate this problem.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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