β3-Tubulin (E9F3E) & CO-0128-647 SignalStar™ Oligo-Antibody Pair #74727
- IHC
Order Information # 74727
This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| SignalStar™ Leica Bond | 1:50 - 1:200 |
| SignalStar™ Manual | 1:50 - 1:200 |
Storage
Product Description
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
β3-tubulin (TUBB3) is one of six β-tubulin isoforms and is expressed highly during fetal and postnatal development (axon guidance and maturation) (2). Its expression levels decrease in the adult central nervous system (CNS) but remain high in the peripheral nervous system (PNS) (3). Microtubules enriched in β3-tubulin are more dynamic than those composed of other β-tubulin isoforms (4). Research studies have shown that mutations in the β3-tubulin gene TUBB3 cause ocular motility defects and other nervous system disorders. Furthermore, β3-tubulin is present in neoplastic but not in normal differentiated glial cells. Thus, β3-tubulin is a great neuronal marker (5).
- Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
- Jiang, Y.Q. and Oblinger, M.M. (1992) J Cell Sci 103 ( Pt 3), 643-51.
- Panda, D. et al. (1994) Proc Natl Acad Sci U S A 91, 11358-62.
- Tischfield, M.A. et al. (2010) Cell 140, 74-87.
- Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.
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