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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7
  1. Products /
  2. SignalStar™ Multiplex IHC Kits & Reagents /
  3. CD3ε (E4T1B) & CO-0048-750 SignalStar™ Oligo-Antibody Pair

CD3ε (E4T1B) & CO-0048-750 SignalStar™ Oligo-Antibody Pair #75727

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  • IHC
Immunohistochemistry Image 1: CD3ε (E4T1B) & CO-0048-750 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) & CO-0042-488 SignalStar™ Oligo-Antibody Pair #95804 (green), CD45 (D3F8Q) & CO-0046-594 SignalStar™ Oligo-Antibody Pair #10373 (yellow), CD3ε (E4T1B) & CO-0048-647 SignalStar™ Oligo-Antibody Pair #47865 (white), CD86 (E5W6H) & CO-0051-750 SignalStar™ Oligo-Antibody Pair #95017 (cyan), TCF1/TCF7 (C63D9) & CO-0006-594 SignalStar™ Oligo-Antibody Pair #34706 (orange), Granzyme B (E5V2L) & CO-0049-647 SignalStar™ Oligo-Antibody Pair #98534 (red), and Ly-6G (E6Z1T) & CO-0053-750 SignalStar™ Oligo-Antibody Pair #64664 (magenta). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
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Order Information # 75727

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
CD3ε (E4T1B) XP® Rabbit mAb (SignalStar™ Conjugate 0048) #6291850 µlMRabbit IgG
Complementary Oligo (CO-0048-750) #8155822 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

CD3ε (E4T1B) XP® Rabbit mAb (SignalStar™ Conjugate 0048) recognizes endogenous levels of total CD3ε protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu50 of mouse CD3ε protein.

Background

When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).

Database Links

UniProt ID: P22646


Entrez-Gene Id: 12501


Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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