CD40 (D8W3N) & CO-0115-750 SignalStar™ Oligo-Antibody Pair #83618
- IHC
Order Information # 83618
This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.
Product Information
Product Usage Information
Application | Dilution |
---|---|
SignalStar™ Leica Bond | 1:50 - 1:200 |
SignalStar™ Manual | 1:50 - 1:200 |
Storage
Product Description
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala135 of human CD40 protein.
Background
CD40, also known as tumor necrosis factor receptor superfamily member 5 (TNFRSF5), is a type I transmembrane protein expressed on the surface of B cells and professional antigen-presenting cells of the immune system, as well as on several non-hematopoietic cell types and cancers (1-4). CD40 interacts with CD40 ligand (CD40L/TNFSF5), which is expressed primarily on activated T cells but has also been reported on blood platelets, mast cells, basophils, NK cells, and B cells (5). Upon engagement with CD40L, CD40 signals through TNF receptor associated factors and MAP kinase signaling pathways, resulting in a wide variety of immune and inflammatory responses, including dendritic cell activation and cross-presentation, T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation (6-8). The CD40/CD40L axis is essential for the initiation and progression of cellular and humoral adaptive immunity, and is an important area of interest in the study of tumor immunology, neurodegenerative diseases, vascular diseases, and inflammatory disorders (9-12).
- Banchereau, J. et al. (1995) Adv Exp Med Biol 378, 79-83.
- Cooke, P.W. et al. (1999) J Pathol 188, 38-43.
- Hakkarainen, T. et al. (2003) Clin Cancer Res 9, 619-24.
- Slobodova, Z. et al. (2011) Neoplasma 58, 189-97.
- Gauchat, J.F. et al. (1993) FEBS Lett 315, 259-66.
- Quezada, S.A. et al. (2004) Annu Rev Immunol 22, 307-28.
- Spriggs, M.K. et al. (1992) J Exp Med 176, 1543-50.
- Elgueta, R. et al. (2009) Immunol Rev 229, 152-72.
- Korniluk, A. et al. (2014) Tumour Biol 35, 9447-57.
- Townsend, K.P. et al. (2005) Eur J Immunol 35, 901-10.
- Hassan, G.S. et al. (2012) Immunobiology 217, 521-32.
- Zhang, B. et al. (2013) Immunol Lett 153, 58-61.
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