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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

CD54/ICAM-1 (E3Q9N) & CO-0146-750 SignalStar Oligo-Antibody Pair #81990

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  • IHC
Immunohistochemistry Image 1: CD54/ICAM-1 (E3Q9N) & CO-0146-750 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human tonsil using Phospho-Histone H2A.X (Ser139) (D7T2V) & CO-0135-488 SignalStar™ Oligo-Antibody Pair #66478 (magenta), CD54/ICAM-1 (E3Q9N) & CO-0146-594 SignalStar™ Oligo-Antibody Pair #32147 (yellow), Myeloperoxidase (E1E7I) & CO-0138-647 SignalStar™ Oligo-Antibody Pair #53259 (red), Pan-Keratin (C11) & CO-0003-750 SignalStar™ Oligo-Antibody Pair #97227 (cyan), CD8α (D8A8Y) & CO-0004-488 SignalStar™ Oligo-Antibody Pair #45747 (green), Ki-67 (8D5) & CO-0014-594 SignalStar™ Oligo-Antibody Pair #35330 (white), and α-Smooth Muscle Actin (D4K9N) & CO-0024-647 SignalStar™ Oligo-Antibody Pair #63902 (orange). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

Order Information # 81990

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
CD54/ICAM-1 (E3Q9N) XP® Rabbit mAb (SignalStar™ Conjugate 0146) #9868750 µlHRabbit IgG
Complementary Oligo (CO-0146-750) #4170022 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

CD54/ICAM-1 (E3Q9N) XP® Rabbit mAb (SignalStar™ Conjugate 0146) recognizes endogenous levels of total CD54/ICAM-1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro410 of human CD54/ICAM-1 protein.

Background

Intercellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. CD54 is expressed at low levels in diverse cell types, and is induced by cytokines (TNF-α, interleukin-1) and bacterial lipopolysaccharide (1). Apical localization of CD54 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier (1). Apical expression of CD54 on epithelial cells mediates pathogen invasion as well as host defense, a pattern also observed in tumors (1). CD54 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 (leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation (2). Cross-linking of CD54 or binding to its ligand triggers activation of Src family kinases and the Rho/ROCK pathway (3-7). Phosphorylation on Tyr485 of CD54 is required for its association with SHP-2 (5). SHP-2 seems essential for CD54-induced Src activation (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a trademark of Cell Signaling Technology, Inc.
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U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.
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