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Product last modified at: 2025-03-06T09:00:19.433Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

F4/80 (D2S9R) & CO-0042-594 SignalStar Oligo-Antibody Pair #24358

Filter:
  • IHC
Immunohistochemistry Image 1: F4/80 (D2S9R) & CO-0042-594 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) & CO-0042-488 SignalStar™ Oligo-Antibody Pair #95804 (green), CD45 (D3F8Q) & CO-0046-594 SignalStar™ Oligo-Antibody Pair #10373 (yellow), CD3ε (E4T1B) & CO-0048-647 SignalStar™ Oligo-Antibody Pair #47865 (white), CD86 (E5W6H) & CO-0051-750 SignalStar™ Oligo-Antibody Pair #95017 (cyan), TCF1/TCF7 (C63D9) & CO-0006-594 SignalStar™ Oligo-Antibody Pair #34706 (orange), Granzyme B (E5V2L) & CO-0049-647 SignalStar™ Oligo-Antibody Pair #98534 (red), and Ly-6G (E6Z1T) & CO-0053-750 SignalStar™ Oligo-Antibody Pair #64664 (magenta). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

Order Information # 24358

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
F4/80 (D2S9R) XP® Rabbit mAb (SignalStar™ Conjugate 0042) #7853850 µlMRabbit IgG
Complementary Oligo (CO-0042-594) #1562522 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

F4/80 (D2S9R) XP® Rabbit mAb (SignalStar™ Conjugate 0042) recognizes endogenous levels of total F4/80 protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant mouse F4/80 protein.

Background

F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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