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Product last modified at: 2025-03-06T09:00:37.371Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

Granzyme B (E5V2L) & CO-0049-488 SignalStar Oligo-Antibody Pair #65622

Filter:
  • IHC
Immunohistochemistry Image 1: Granzyme B (E5V2L) & CO-0049-488 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) & CO-0042-488 SignalStar™ Oligo-Antibody Pair #95804 (green), CD45 (D3F8Q) & CO-0046-594 SignalStar™ Oligo-Antibody Pair #10373 (yellow), CD3ε (E4T1B) & CO-0048-647 SignalStar™ Oligo-Antibody Pair #47865 (white), CD86 (E5W6H) & CO-0051-750 SignalStar™ Oligo-Antibody Pair #95017 (cyan), TCF1/TCF7 (C63D9) & CO-0006-594 SignalStar™ Oligo-Antibody Pair #34706 (orange), Granzyme B (E5V2L) & CO-0049-647 SignalStar™ Oligo-Antibody Pair #98534 (red), and Ly-6G (E6Z1T) & CO-0053-750 SignalStar™ Oligo-Antibody Pair #64664 (magenta). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

Order Information # 65622

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
Granzyme B (E5V2L) Rabbit mAb (SignalStar™ Conjugate 0049) #8424650 µlMRabbit IgG
Complementary Oligo (CO-0049-488) #9961122 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

Granzyme B (E5V2L) Rabbit mAb (SignalStar™ Conjugate 0049) recognizes endogenous levels of total mouse Granzyme B protein. This antibody does not cross-react with human Granzyme B proteins. Non-specific staining was observed in mouse kidney by immunohistochemistry.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala197 of mouse Granzyme B protein.

Background

Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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