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Product last modified at: 2025-03-06T09:00:33.062Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

Ki-67 (8D5) & CO-0014-488 SignalStar Oligo-Antibody Pair #89034

Filter:
  • IHC
Immunohistochemistry Image 1: Ki-67 (8D5) & CO-0014-488 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Ki-67 (8D5) & CO-0014-488 SignalStar™ Oligo-Antibody Pair #89034 (green), Pan-Keratin (C11) & CO-0003-488 SignalStar™ Oligo-Antibody Pair #63566 (magenta), CD31 (PECAM-1) (89C2) & CO-0028-594 SignalStar™ Oligo-Antibody Pair #33420 (yellow), Vimentin (D21H3) & CO-0012-594 SignalStar™ Oligo-Antibody Pair #16471 (orange), CD11c (D3V1E) & CO-0017-647 SignalStar™ Oligo-Antibody Pair #96411 (red), TCF1/TCF7 (C63D9) & CO-0006-647 SignalStar™ Oligo-Antibody Pair #25502 (pink), CD8α (D8A8Y) & CO-0004-750 SignalStar™ Oligo-Antibody Pair #62750 (cyan), and CD3ε (D7A6E™) & CO-0001-750 SignalStar™ Oligo-Antibody Pair #51754 (white). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.

Order Information # 89034

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
Ki-67 (8D5) Mouse mAb (SignalStar™ Conjugate 0014) #6390650 µlHMouse IgG1
Complementary Oligo (CO-0014-488) #6873522 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

Ki-67 (8D5) Mouse mAb (SignalStar™ Conjugate 0014) recognizes endogenous levels of total Ki-67 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Ki-67 protein.

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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