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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

LAG3 (D2G4O) & CO-0026-647 SignalStar Oligo-Antibody Pair #40966

Filter:
  • IHC
Immunohistochemistry Image 1: LAG3 (D2G4O™) & CO-0026-647 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human tonsil using LAG3 (D2G4O™) & CO-0026-647 SignalStar™ Oligo-Antibody Pair #40966 (magenta), PD-L1 (E1L3N®) & CO-0005-488 SignalStar™ Oligo-Antibody Pair #85646 (green), PD-1 (Intracellular Domain) (D4W2J) & CO-0008-594 SignalStar™ Oligo-Antibody Pair #35347 (yellow), TIM-3 (D5D5R™) & CO-0010-647 SignalStar™ Oligo-Antibody Pair #15231 (red), Tox/Tox2 (E6I3Q) & CO-0016-750 SignalStar™ Oligo-Antibody Pair #81643 (cyan), CD3ε (D7A6E™) & CO-0001-488 SignalStar™ Oligo-Antibody Pair #92856 (pink), CD8α (D8A8Y) & CO-0004-594 SignalStar™ Oligo-Antibody Pair #19166 (orange), Pan-Keratin (C11) & CO-0003-750 SignalStar™ Oligo-Antibody Pair #97227 (white), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.

Order Information # 40966

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
LAG3 (D2G4O™) XP® Rabbit mAb (SignalStar™ Conjugate 0026) #9491450 µlHRabbit IgG
Complementary Oligo (CO-0026-647) #1919822 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

LAG3 (D2G4O™) XP® Rabbit mAb (SignalStar™ Conjugate 0026) recognizes endogenous levels of total LAG3 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LAG3 protein.

Background

Lymphocyte activation gene 3 (LAG3, CD223) is an immune checkpoint control protein that negatively regulates T cells and immune responses. A CD4-like member of the Ig superfamily, LAG3 contains an extracellular IgV and three IgC domains, a transmembrane domain, and a short cytoplasmic region (1). LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, Tregs, and NK cells, where it's activated by MHC Class II molecules, its only known ligand. While it was initially shown to activate Treg cells (2), LAG3 can also inhibit CD8+ T cells (3,4). LAG3 is often co-expressed with PD-1 on the surface of tumor infiltrating lymphocytes, where the two proteins act independently to contribute to tumor-mediated immune suppression (4,5). Blockade of LAG3 is a promising strategy for neoplastic intervention (6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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