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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7
  1. Products /
  2. SignalStar™ Multiplex IHC Kits & Reagents /
  3. Mesothelin (D9R5G) & CO-0169-750 SignalStar™ Oligo-Antibody Pair

Mesothelin (D9R5G) & CO-0169-750 SignalStar™ Oligo-Antibody Pair #79723

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  • IHC
Immunohistochemistry Image 1: Mesothelin (D9R5G) & CO-0169-750 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using Mesothelin (D9R5G) Rabbit mAb (647; magenta), CD3ε (D7A6E) Rabbit mAb (488; green), Ki-67 (8D5) Mouse mAb (488; yellow), CD11c (D3V1E) Rabbit mAb (594; red), α-Smooth Muscle Actin (D4K9N) Rabbit mAb (647; cyan), CD31 (PECAM-1) (89C2) Mouse mAb (750; white), and DAPI #4083 (blue). Staining was performed on the BOND RX autostainer by Leica Biosystems.
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Order Information # 79723

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
Product IncludesVolumeReactivityIsotype
Mesothelin (D9R5G) XP® Rabbit mAb (SignalStar™ Conjugate 0169) #1671750 µlHRabbit IgG
Complementary Oligo (CO-0169-750) #2928730 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

Mesothelin (D9R5G) XP® Rabbit mAb (SignalStar® Conjugate 0169) recognizes endogenous levels of total mesothelin protein. The antibody detects both uncleaved (full-length) and cleaved forms of mesothelin, but does not detect the cleaved fragment corresponding to megakaryocyte-potentiating factor (MPF).

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human mesothelin protein.

Background

The MSLN gene encodes a 69 kDa precursor protein that is proteolytically cleaved to yield megakaryocyte potentiating factor (MPF) and a GPI-anchored membrane protein termed mesothelin (1). Expression of (cleaved) mesothelin is largely confined to mesothelial cells of normal pleura, pericardium, and peritoneum, but has been reported to be overexpressed in some cancers, including mesothelioma, and some pancreatic and ovarian adenocarcinomas (1,2). Although suggested to be involved in cell adhesion, the physiological functions of mesothelin have not been determined. It is known, however, that mesothelin can be shed from the cell surface following cleavage by TNF-α converting enzyme. Research studies show that serum levels of mesothelin are markedly increased in patients with mesothelioma and ovarian cancer (1), suggesting that serum mesothelin levels may have utility as a cancer biomarker (1-3).

Database Links

UniProt ID: Q13421


Entrez-Gene Id: 10232


Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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