Render Target: STATIC
Render Timestamp: 2025-01-20T13:16:58.466Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2025-01-08 16:43:12.130
Product last modified at: 2025-01-01T09:05:44.796Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

Pan-Keratin (C11) & CO-0003-594 SignalStar Oligo-Antibody Pair #30989

Filter:
  • IHC
Immunohistochemistry Image 1: Pan-Keratin (C11) & CO-0003-594 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using Pan-Keratin (C11) & CO-0003-488 SignalStar™ Oligo-Antibody Pair #63566 (red), PD-L1 (E1L3N®) & CO-0005-488 SignalStar™ Oligo-Antibody Pair #85646 (green), PD-1 (Intracellular Domain) (D4W2J) & CO-0008-594 SignalStar™ Oligo-Antibody Pair #35347 (yellow), Granzyme B (D6E9W) & CO-0009-594 SignalStar™ Oligo-Antibody Pair #15194 (orange), TIGIT (E5Y1W) & CO-0002-647 SignalStar™ Oligo-Antibody Pair #18288 (cyan), CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair #36775 (pink), CD3ε (D7A6E™) & CO-0001-750 SignalStar™ Oligo-Antibody Pair #51754 (magenta), CD8α (D8A8Y) & CO-0004-750 SignalStar™ Oligo-Antibody Pair #62750 (white), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.

Order Information # 30989

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
Pan-Keratin (C11) Mouse mAb (SignalStar™ Conjugate 0003) #3296250 µlHMouse IgG1
Complementary Oligo (CO-0003-594) #7118122 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

Pan-Keratin (C11) Mouse mAb (SignalStar™ Conjugate 0003) detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13, and 18. The antibody does not cross-react with other keratins.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A-431 cells.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins K9-K28) and a basic keratin (or type II keratin, keratins K1-K8 and K71-K80) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research and clinical biomarkers (1,2).

Dysregulation/mutations in keratin genes can lead to a variety of disorders affecting the skin, hair, nails, and other epithelial tissues (3). While expression of keratins can be variable, immunohistochemical staining of keratins is widely used to help in the identification and classification of epithelial tumors, and may also provide prognostic information.

Keratins 8 and 18 (K8/K18) are expressed in simple epithelia of normal tissue, as well as in adenocarcinomas of the breast, lung, ovary, and gastrointestinal tract. Keratin 17 is expressed in basal keratinocytes of stratified epithelia, hair follicles, and sebaceous glands. Onset of keratin 17 expression coincides with the definition of major epithelial lineages during skin development (4). Keratin 14 (K14) is expressed in basal cells of stratified epithelia, and in basal-like subtypes of breast cancer and squamous cell carcinomas. Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas. Keratin 5/6 (K5/6) is expressed in basal cells of stratified epithelia, including the skin, prostate, and breast, as well as in basal-like breast cancers, squamous cell carcinomas, and some lung carcinomas. Keratin 7 (K7) is expressed in glandular epithelia, such as those in the lung, breast, and female reproductive tract, as well as in adenocarcinomas of the lung, breast, and ovary (5,6).

Keratins, particularly K8, K18, and K19, serve as biomarkers for identification of circulating tumor cells (CTCs) (5).

Post-translational modifications, including phosphorylation, acetylation, ubiquitylation, sumoylation, glycosylation, and transamidation, have been shown to affect the functions of keratins in normal and disease states (6). Understanding the molecular mechanisms underlying these PTMs may provide insights into cancer pathogenesis.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.