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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

Phospho-CREB (Ser133) (87G3) & CO-0125-750 SignalStar Oligo-Antibody Pair #97252

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  • IHC
Immunohistochemistry Image 1: Phospho-CREB (Ser133) (87G3) & CO-0125-750 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using c-Fos (E2I7R) & CO-0131-488 SignalStar™ Oligo-Antibody Pair #28962 (green), α-Smooth Muscle Actin (D4K9N) & CO-0024-594 SignalStar™ Oligo-Antibody Pair #25040 (magenta), and Phospho-CREB (Ser133) (87G3) & CO-0125-647 SignalStar™ Oligo-Antibody Pair #84641 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

Order Information # 97252

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
Phospho-CREB (Ser133) (87G3) Rabbit mAb (SignalStar™ Conjugate 0125) #5799250 µlH M RRabbit IgG
Complementary Oligo (CO-0125-750) #2009822 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

Phospho-CREB (Ser133) (87G3) Rabbit mAb (SignalStar™ Conjugate 0125) detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

Pathways

Explore pathways related to this product.


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U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.
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