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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

Phospho-Stat3 (Tyr705) (D3A7) & CO-0029-488 SignalStar Oligo-Antibody Pair #74891

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  • IHC
Immunohistochemistry Image 1: Phospho-Stat3 (Tyr705) (D3A7) & CO-0029-488 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using Phospho-Stat3 (Tyr705) (D3A7) & CO-0029-594 SignalStar™ Oligo-Antibody Pair #86323 (yellow), Pan-Keratin (C11) & CO-0003-488 SignalStar™ Oligo-Antibody Pair #63566 (green), Tox/Tox2 (E6I3Q) & CO-0016-488 SignalStar™ Oligo-Antibody Pair #31189 (magenta), α-Smooth Muscle Actin (D4K9N) & CO-0024-647 SignalStar™ Oligo-Antibody Pair #63902 (red), CD163 (D6U1J) & CO-0022-750 SignalStar™ Oligo-Antibody Pair #71043 (cyan), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.

Order Information # 74891

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (SignalStar™ Conjugate 0029) #5619250 µlH M R MkRabbit IgG
Complementary Oligo (CO-0029-488) #1757622 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (SignalStar™ Conjugate 0029) detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

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