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BCA Protein Assay Kit

BCA Protein Assay Kit #7780

Name: BCA Protein Assay Kit
Price: 375.00 USD
Availability: InStock

Product Information

Product Usage Information

Storage

Store at room temperature.

Protocol

Product Description

The BCA Protein Assay Kit can be used to measure the protein concentration of lysates or homogenates, in microplate format, prepared with the following buffers: Cell Lysis Buffer (10X) #9803, RIPA Buffer (10X) #9806, PathScan^® Sandwich ELISA Lysis Buffer (1X) #7018. The dynamic range for this assay is 0.125 - 2 mg/mL. It is recommended that the BCA Compatibility Reagent be used to decrease interference from reducing agents, chelators, detergents, and other common ingredients found in most lysis buffers. Please see the attached protocol for additional details.

Background

Bicinchoninic Acid (BCA) is capable of forming an intense purple complex with cuprous ion, Cu¹⁺, in an alkaline environment. Cu^1h+ is produced from the reaction of protein with alkaline Cu²⁺. The resulting reaction and color produced is the basis for a common protein quantification method capable of measuring protein concentration over a wide range. Increasing protein concentrations produce proportionally deeper colors. The BCA protein assay demonstrates higher tolerances towards common interfering substances, such as nonionic detergents and buffer salts, than the Lowry technique (1).

Smith, P.K. et al. (1985) Anal Biochem 150, 76-85.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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