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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c

His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate) #8811

Filter:
  • IP
Immunoprecipitation Image 1: His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate)
Immunoprecipitation of extracts from COS-7 cells transfected with a construct expressing a His-tagged protein (Tyro3-His), using Mouse IgG (Magnetic Bead Conjugate) #5873 (lane 2) or His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate) (lane 3). Lane 1 is 10% input. The western blot analysis was performed using His-Tag Antibody #2365.

To Purchase # 8811

Cat. # Size Qty. Price Ships
8811S 400 µl
$450

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Mouse IgG1
Application Key:
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • All-All Species Expected 

Product Information

Product Description

This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation of recombinant proteins containing the 6xHis epitope tag.

Product Usage Information

Application Dilution
Immunoprecipitation 1:20

Storage

Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate) detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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