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Render Timestamp: 2024-12-23T12:25:31.901Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:19:20.379
Product last modified at: 2024-11-15T14:30:12.265Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. WB & IP Reagents /
  3. Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Sepharose® Bead Conjugate)

Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Sepharose® Bead Conjugate) #9219

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  • IP

    Supporting Data

    REACTIVITY H M R Mk Sc
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Mouse IgG1
    Application Key:
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Sc-S. cerevisiae 

    Product Information

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:20

    Solutions and Reagents

    Storage Buffer:20 mM Tris-HCl (pH 7.4)150 mM NaCl1 mM Na2EDTA1 mM EGTA1% Triton X-1002.5 mM sodium pyrophosphate1 mM β-glycerophosphate1 mM Na3VO41 µg/ml leupeptin50% glycerolNote: Please aliquot prior to first use.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose® Bead Conjugate) binds only p38 MAPK only when dually phosphorylated at Thr180 and Tyr182. This antibody was immobilized via conjugation of carbohydrates to cross-linked agarose hydrazide beads. This antibody does not significantly cross-react with phosphorylated SAPK/JNK or p44/42 MAPK, or with nonphosphorylated p38 MAPK.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, S. cerevisiae

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Zebrafish

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.

    Background

    p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

    Database Links

    UniProt ID: Q16539 , O15264 , P53778 , Q15759


    Entrez-Gene Id: 1432 , 5603 , 6300 , 5600


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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