Render Target: STATIC
Render Timestamp: 2024-12-03T11:21:10.896Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-20 06:18:57.933
Product last modified at: 2024-11-25T17:15:08.079Z
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PDP - Template Name: Monoclonal Antibody (Isotype Control)
PDP - Template ID: *******4b30f73
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) #8726

Filter:
  • IP

    Supporting Data

    Concentration 10 mg/ml
    SENSITIVITY Endogenous
    Source/Isotype Rabbit IgG
    Application Key:
    • IP-Immunoprecipitation 

    Product Information

    Product Description

    This Cell Signaling Technology (CST) antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.

    Product Usage Information

    Directions for Use: Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

    For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.

    Application Dilution
    Immunoprecipitation 1:20

    Storage

    Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibodies.

    Specificity / Sensitivity

    Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.

    Background

    Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
      XP is a registered trademark of Cell Signaling Technology, Inc.
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