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Western Blot Stripping Buffer (5X)

Western Blot Stripping Buffer (5X) #91925

Name: Western Blot Stripping Buffer (5X)
Price: 127.00 USD
Availability: InStock

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Product Information

Storage

Store Western Blot Stripping Buffer (5X) at room temperature. This product is stable for at least 12 months.

Protocol

Product Description

Western Blot Stripping Buffer (5X) allows for quick and effective removal of both primary and secondary antibodies from nitrocellulose membranes. This can be done without damaging the immobilized antigen, enabling the membranes to be reprobed with alternative antibodies or concentrations for further western blot analysis. This product should be diluted to 1X using RODI-water, and 2-mercaptoethanol should be added right before use. Completely submerge the membrane in Western Blot Stripping Buffer (1X). Optimization of incubation time or temperature may be required to achieve desired results.

Background

Having to re-run gels and repeat western blotting efforts is costly and time-consuming. Western Blot Stripping Buffer (5X) enables the membranes to be reused with the added benefit of not having to re-load expensive or limited samples into the gel. Allowing for the detection of a different target with an alternative primary antibody or by reprobing with a different concentration of a primary antibody saves valuable time.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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