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YAP - Mediator of the Hippo Signaling Pathway

YAP (Yes-associated protein, YAP65) is a transcriptional co-activator that functions as the primary effector of the Hippo tumor suppressor pathway. Hippo signaling via YAP regulates tissue growth and organ size, in part by modulating the proliferative activity of stem and progenitor cells. YAP is a potent oncogene whose aberrant regulation can lead to tumorigenesis, and elevated YAP expression has been reported in many human tumor types. Research studies also suggest that YAP plays an important role in cell fate determination. In addition to its reported ability to promote epithelial-mesenchymal transition (1), YAP was shown to promote dedifferentiation of mature hepatocytes into hepatic progenitor cells, suggesting an important role for YAP and Hippo signaling in tissue regeneration (2).

  1. Lamar JM, Stern P, Liu H, Schindler JW, Jiang ZG, Hynes RO (2012) The Hippo pathway target, YAP, promotes metastasis through its TEAD-interaction domain. Proc. Natl. Acad. Sci. U.S.A. 109(37), E2441–50.
  2. Yimlamai D, Christodoulou C, Galli GG, Yanger K, Pepe-Mooney B, Gurung B, Shrestha K, Cahan P, Stanger BZ, Camargo FD (2014) Hippo pathway activity influences liver cell fate. Cell 157(6), 1324–38.

Antibody Performance Comparisons

We compared our YAP (D8H1X) XP® Rabbit mAb #14074 with 3 YAP antibodies from other companies and found that it performed as well or better in every application tested. See competitor comparison data below for individual applications.

Western Blotting

Western blot analysis of extracts from YAP positive cells (A-204, A549, MCF7) and YAP negative cells (RL) using our YAP (D8H1X) XP® Rabbit mAb #14074, and three other antibodies at the recommended dilutions.

Western Blotting Competitor Comparison with YAP #14074
  • YAP (D8H1X) XP® Rabbit mAb #14074 results in a band at the appropriate molecular weight for YAP in YAP positive cells and no bands in YAP negative cells, indicating that it is sensitive and specific for YAP.
  • Antibody 1 gives a strong signal for YAP, but strongly cross-reacts with a 50 kDa protein suggesting that this antibody is not specific for YAP.
  • Antibody 2 produces a weak signal for YAP at the dilution recommended by the supplier.
  • Antibody 3 gives bands at multiple molecular weights for both YAP positive and YAP negative cell lines, demonstrating that this antibody is not specific for YAP.

Summary: YAP (D8H1X) XP® Rabbit mAb #14074 demonstrates higher specificity and sensitivity than the other antibodies.

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