Motif Antibodies for PTMScan^® Discovery Services

Cell Signaling Technology developed Motif Antibodies to enable focused analysis of regulatory post-translational modifications (PTMs). We use these in the form of custom PTMScan License/IAP Bead Products in our PTMScan Discovery Services, and we provide these products with a Per-Use License to enable our customers to perform PTMScan proteomics in-house.

Whether your research objective is to understand specific serine/threonine kinase families or kinome branches, global tyrosine phosphorylation, protein acetylation, or ubiquitin-mediated signaling, our PTMScan Discovery Services and PTMScan License/IAP Beads products provide powerful tools for in-depth proteomic analysis of regulatory PTMs.

PTMScan – PhosphoScan^®, UbiScan^®, AcetylScan^® – is the most effective strategy for PTM proteomics, and our PTMScan License/IAP Bead products (see below) are custom, optimized reagents for immunoaffinity purification (IAP) of serine/threonine and tyrosine phosphopeptides, acetylated peptides and ubiquitin-tagged peptides. PTMScan is a method that combines Motif Antibody-based IAP and tandem mass spectrometry to generate focused and quantitative results about proteins on which PTMs perform key regulatory functions. It is the PTM-specificity of our Motif Antibodies that makes PTMScan Discovery Services and Per-Use Licensing of PTMScan License/IAP Bead products the standards for proteomic analysis of regulatory signaling.

For each of the motif antibodies listed in the table below, a RAW LC-MS/MS data file is provided. The LC-MS/MS data file was generated using a Thermo Orbitrap (Velos or Elite) mass spectrometer. In addition, a result table (in excel format) is accompanied with each RAW data file generated from the corresponding motif antibody enrichment of mouse liver peptides. The peptide/protein assignments in the accompanying excel table were produced using SEQUEST. The sample description for each data set is provided in the header of the results table.