PTMScan® Direct Ser/Thr Kinases Service allows access to perform targeted screening and quantification of a defined set of protein sites within the Ser/Thr kinome critical for drug discovery, biomarker discovery, or diagnostic development. PTMScan Direct Ser/Thr Kinases Service provides kinome-wide assessment of Ser/Thr phosphorylation. The table below illustrates the list of protein sites that can be monitored with PTMScan® Direct Ser/Thr Kinases Service.
Altogether, the PTMScan® Direct Serine/Threonine Kinase service provides a robust method to qualitatively identify and quantitatively profile 385 unique phosphorylation sites to 130 proteins.
| Ser/Thr Protein Sites | |||
|---|---|---|---|
| Ser/Thr Protein Sites | |||
| β-Actin* | Akt1 (Ser473) | Akt1 (Ser475) | Akt1 (Thr305) |
| Akt1 (Thr308) | Akt1 (Thr312) | Akt1 (Thr479) | Akt1 (Tyr315) |
| Akt1 (Tyr474) | Akt2 (Ser302) | Akt2 (Ser474) | Akt2 (Ser476) |
| Akt2 (Ser478) | Akt2 (Thr306) | Akt2 (Thr309) | Akt2 (Thr313) |
| Akt2 (Tyr316) | Akt2 (Tyr475) | Akt3 (Ser472) | Akt3 (Ser474) |
| Akt3 (Ser476) | Akt3 (Thr302) | Akt3 (Thr305) | Akt3 (Thr309) |
| Akt3 (Tyr473) | AMPKα1 (Ser172) | AMPKα1 (Ser176) | AMPKα1 (Ser184) |
| AMPKα1 (Ser187) | AMPKα1 (Ser486) | AMPKα1 (Ser494) | AMPKα1 (Ser496) |
| AMPKα1 (Thr183) | AMPKα1 (Thr482) | AMPKα1 (Thr488) | AMPKα1 (Thr490) |
| AMPKα2 (Ser161) | AMPKα2 (Ser165) | AMPKα2 (Ser173) | AMPKα2 (Ser176) |
| AMPKα2 (Thr172) | ASK1 (Thr916) | ASK1 (Thr918) | ATM (Ser1981) |
| ATM (Ser1983) | Aurora A (Thr287) | Aurora A (Thr288) | Aurora A (Thr292) |
| Aurora B (Thr232) | Aurora B (Thr236) | Aurora C (Thr198) | Aurora C (Thr202) |
| Bcr (Tyr177) | BMPR2 (Thr977) | BMPR2 (Tyr979) | Brd2 (Ser253) |
| Brd2 (Ser256) | Brd4 (Thr942) | CDK1 (Thr14) | CDK1 (Tyr15) |
| CDK1 (Tyr19) | CDK2 (Thr14) | CDK2 (Tyr15) | CDK2 (Tyr19) |
| CDK3 (Thr14) | CDK3 (Tyr15) | CDK3 (Tyr19) | CDK5 (Tyr15) |
| CDK6 (Tyr24) | ChaK1 (Ser1404) | ChaK1 (Ser1407) | CHED (Ser1225) |
| CHED (Ser1229) | CHED (Thr1226) | Chk1 (Ser296) | Chk1 (Ser305) |
| Chk1 (Ser307) | Chk1 (Ser315) | Chk1 (Ser316) | Chk1 (Ser317) |
| Chk1 (Ser343) | Chk1 (Ser345) | Chk1 (Ser357) | Chk1 (Thr348) |
| Chk1 (Thr362) | Chk1 (Tyr314) | CKB (Thr35) | CLK3 (Ser157) |
| CLK3 (Ser165) | CLK3 (Tyr155) | CLK3 (Tyr163) | CLK3 (Tyr166) |
| CRK7 (Thr1244) | CRK7 (Thr1246) | dCK (Ser13) | Erk1 (Thr202) |
| Erk1 (Thr207) | Erk1 (Tyr204) | Erk2 (Thr185) | Erk2 (Thr190) |
| Erk2 (Tyr187) | Erk3 (Ser363) | Erk3 (Tyr357) | Erk5 (Thr218) |
| Erk5 (Tyr220) | GAPDH* | GCN2 (Ser254) | GCN2 (Ser258) |
| GCN2 (Ser261) | GCN2 (Ser264) | GCN2 (Ser274) | GCN2 (Tyr253) |
| GCN2 (Tyr269) | GSK-3α (Ser20) | GSK-3α (Ser21) | GSK-3α (Thr19) |
| GSK-3β (Ser13) | GSK-3β (Ser25) | GSK-3β (Ser9) | GSK-3β (Thr8) |
| HGK (Ser264) | HGK (Thr187) | HKDC1 (Ser155) | HKDC1 (Thr153) |
| Histone H3* | ICK (Thr157) | ICK (Tyr159) | IKKε (Ser172) |
| KSR (Ser265) | KSR (Thr268) | KSR (Thr272) | KSR2 (Thr269) |
| KSR2 (Thr276) | LOC100133591 (Ser223) | LOC651610 (Ser1859) | LOC651610 (Ser1861) |
| LRRK1 (Thr30) | LRRK2 (Ser961) | MAPKAPK-2 (Thr221) | MAPKAPK-2 (Thr222) |
| MAPKAPK-2 (Thr317) | MAPKAPK-2 (Thr329) | MAPKAPK-2 (Thr334) | MAPKAPK-2 (Thr338) |
| MAPKAPK-2 (Tyr225) | MAPKAPK-3 (Ser307) | MAPKAPK-3 (Thr313) | MAPKAPK-3 (Thr317) |
| MARK1 (Thr504) | MARK2 (Ser592) | MARK2 (Ser593) | MARK2 (Ser595) |
| MARK2 (Thr596) | MARK3 (Thr530) | MEK1 (Ser218) | MEK1 (Ser222) |
| MEK1 (Thr226) | MEK2 (Ser222) | MEK2 (Ser226) | MEK2 (Thr230) |
| MINK (Thr187) | MKK3 (Ser212) | MKK3 (Ser218) | MKK3 (Ser253) |
| MKK3 (Thr222) | MKK3 (Tyr214) | MKK3 (Tyr230) | MKK4 (Ser257) |
| MKK4 (Thr261) | MKK6 (Ser201) | MKK6 (Ser207) | MKK6 (Thr211) |
| MSK1 (Ser376) | MSK1 (Ser381) | MSK1 (Tyr375) | MSK2 (Ser360) |
| MSK2 (Ser365) | MSK2 (Ser634) | MSK2 (Thr376) | MSK2 (Tyr359) |
| Mst1 (Ser40) | Mst1 (Ser43) | Mst1 (Tyr41) | Mst3 (Thr182) |
| Mst3 (Thr184) | Mst3 (Thr190) | Mst3 (Thr194) | Mst4 (Thr178) |
| Mst4 (Thr182) | mTOR (Ser178) | mTOR (Ser2442) | mTOR (Ser2448) |
| mTOR (Ser2450) | mTOR (Ser2454) | mTOR (Thr2444) | mTOR (Thr2446) |
| mTOR (Tyr2449) | NDK7 (Ser304) | NDK7 (Thr300) | NDK7 (Thr318) |
| NDK7 (Tyr303) | NDR1 (Thr444) | NDR1 (Tyr445) | NDR2 (Thr442) |
| NDR2 (Tyr443) | OSR1 (Thr185) | OSR1 (Thr189) | p38α (Thr175) |
| p38α (Thr180) | p38α (Tyr182) | p38γ (Thr183) | p38γ (Tyr185) |
| p38δ (Thr180) | p38δ (Tyr182) | p70 S6 Kinase (Ser243) | p70 S6 Kinase (Ser441) |
| p70 S6 Kinase (Ser447) | p70 S6 Kinase (Ser452) | p70 S6 Kinase (Thr248) | p70 S6 Kinase (Thr250) |
| p70 S6 Kinase (Thr252) | p70 S6 Kinase (Thr444) | p70 S6 Kinase β (Ser219) | p70 S6 Kinase β (Thr226) |
| p70 S6 Kinase β (Thr228) | p90RSK (Ser221) | p90RSK (Ser380) | p90RSK (Thr225) |
| p90RSK (Tyr220) | PAK4 (Ser474) | PAK4 (Thr207) | PAK4 (Thr478) |
| PAK5 (Ser602) | PAK6 (Ser560) | PCTAIRE1 (Ser138) | PCTAIRE1 (Tyr176) |
| PCTAIRE2 (Tyr203) | PDK1 (Ser241) | PDK1 (Thr245) | PDK1 (Tyr248) |
| PDPK2 (Ser214) | PDPK2 (Thr218) | PDPK2 (Tyr221) | PHKA2 (Ser1015) |
| PHKA2 (Ser1022) | PHKA2 (Ser691) | PI3 Kinase p110-alpha (Ser507) | PITSLRE B (Tyr437) |
| PITSLRE (Thr595) | PITSLRE (Tyr449) | PKA C-α (Thr196) | PKA C-α (Thr198) |
| PKA C-α (Thr202) | PKA C-β (Thr196) | PKA C-β (Thr198) | PKA C-β (Thr202) |
| PKA C-γ (Thr196) | PKA C-γ (Thr198) | PKA C-γ (Thr202) | PKCα (Ser226) |
| PKCα (Ser657) | PKCα (Thr494) | PKCα (Thr495) | PKCα (Thr497) |
| PKCα (Thr501) | PKCα (Tyr504) | PKCβ (Thr497) | PKCβ (Thr500) |
| PKCβ (Thr504) | PKCδ (Ser304) | PKCδ (Ser506) | PKCδ (Ser645) |
| PKCδ (Ser647) | PKCδ (Ser654) | PKCδ (Ser658) | PKCδ (Ser664) |
| PKCδ (Thr507) | PKCδ (Thr511) | PKCδ (Tyr313) | PKCδ (Tyr334) |
| PKCδ (Tyr514) | PKCδ (Tyr646) | PKCε (Ser729) | PKCγ (Thr510) |
| PKCγ (Thr511) | PKCγ (Thr514) | PKCγ (Thr518) | PKCι (Ser411) |
| PKCι (Thr409) | PKCι (Thr410) | PKCι (Thr412) | PKCι (Thr416) |
| PKCθ (Thr307) | PKCζ (Ser409) | PKCζ (Thr407) | PKCζ (Thr410) |
| PKM2 (Ser77) | PKN1 (Ser495) | PKN1 (Ser773) | PKN1 (Thr772) |
| PKN1 (Thr774) | PKN1 (Tyr768) | PKN2 (Ser497) | PKN2 (Ser815) |
| PKN2 (Thr814) | PKN2 (Thr816) | PKN2 (Thr820) | PKN2 (Tyr810) |
| PKN3 (Thr718) | PLK1 (Thr210) | PLK1 (Thr214) | PRP4 (Ser852) |
| PRP4 (Tyr849) | PRPS1 (Ser314) | PRPS1L1 (Ser314) | PRPS2 (Ser314) |
| PYGB (Ser15) | PYGB (Tyr263) | A-Raf (Ser299) | A-Raf (Tyr296) |
| A-Raf (Tyr301) | A-Raf (Tyr302) | B-Raf (Ser446) | B-Raf (Ser447) |
| B-Raf (Thr440) | Raf1 (Ser338) | Raf1 (Ser339) | Raf1 (Tyr340) |
| Raf1 (Tyr341) | RIOK2 (Ser332) | RIOK2 (Ser335) | RIOK2 (Tyr366) |
| RIPK2 (Ser539) | RSK2 (Ser227) | RSK2 (Ser386) | RSK2 (Thr231) |
| RSK2 (Tyr226) | RSK4 (Ser232) | RSK4 (Thr236) | RSK4 (Tyr231) |
| JNK1 (Ser179) | JNK1 (Thr183) | JNK1 (Thr188) | JNK1 (Tyr185) |
| JNK2 (Thr183) | JNK2 (Thr188) | JNK2 (Tyr185) | JNK3 (Ser217) |
| JNK3 (Thr221) | JNK3 (Thr226) | JNK3 (Tyr223) | SGK1 (Ser74) |
| SGK1 (Ser78) | SGK1 (Ser80) | SGK2 (Thr251) | SGK2 (Thr253) |
| SgK223 (Thr203) | SGK3 (Thr320) | SGK3 (Thr324) | smMLCK (Thr276) |
| SPEG (Ser378) | SPEG (Ser381) | SPEG (Thr375) | SPAK (Thr233) |
| SPAK (Thr237) | TAO1 (Ser181) | TAO1 (Thr185) | TAO2 (Ser181) |
| TAO3 (Ser177) | TAO3 (Thr181) | TBK1 (Ser172) | TIF1β (Ser459) |
| TIF1β (Ser460) | TIF1β (Tyr458) | TNIK (Thr187) | TTK (Thr33) |
| α-Tubulin* | ULK1 (Ser556) | WNK1 (Ser1959) | YSK1 (Thr166) |
| YSK1 (Thr168) | YSK1 (Thr174) | YSK1 (Thr178) | ZAK (Ser591) |
| ZAK (Ser610) | ZAK (Ser648) | ZAK (Ser649) | ZAK (Thr576) |
| ZAK (Thr742) | |||
* The following targets are included as control markers for total protein: β-Actin, Histone H3, GAPDH, and α-Tubulin.
