Immunohistochemistry Technical Support

CST is committed to supporting the diverse needs of the research community. Our team has worked diligently to offer over 700 recombinant monoclonal antibodies, rigorously validated for immunohistochemistry (IHC) using FFPE tissues. This allows our scientists to provide expert advice that will save you time and accelerate your results. Our extensive validation ensures that all of our IHC-P validated antibodies can be used for fluorescent multiplex IHC.

Contact our advanced technical support team to answer questions with your assay, including detailed information on:

• IHC validation
• IHC assay troubleshooting
• Leica BOND autostainer protocol development
• Multiplex IHC assay protocols and troubleshooting

To ensure a timely solution please have the relevant information below ready when you contact us.

Have you consulted our Immunohistochemistry Troubleshooting Guide before using this technical report?

Tissue

1. Species.
2. Organ.
3. Fixation (drop fixed, perfused, or unfixed).
   1. Fixative used
   2. Time
   3. Temperature
4. Sections (paraffin-embedded, fixed cryosections, fresh frozen, floating).

Antigen Retrieval

1. Buffer.
2. Describe procedure (time, temperatures, apparatus, etc.).

Hydrogen Peroxide Quench Performed?

1. Buffer.
2. Time.

Block

1. Buffer.
2. Time.
3. Temperature.

Primary Antibody

1. Catalog number.
2. Lot number and reference date.
3. Dilution.
4. Diluent.
5. Length of incubation.
6. Temperature of incubation.

Wash Buffer

Secondary Reagents

1. Manufacturer.
2. Dilution.
3. Time.
4. Temperature.

Substrate

1. Name.
2. Length of incubation.

Results

1. Localization/background.

Controls

1. Negative used and result.
2. Positive used and result.

No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.