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Chromatin Profiling Just Got Faster with CUT&Tag

 

CUT&Tag (Cleavage Under Targets and Tagmentation) is a novel chromatin profiling technique that utilizes pAG-Tn5, a primary antibody, and a secondary antibody to unravel protein-DNA interactions across the genome. Unlike ChIP-seq, CUT&Tag does not rely on immunoprecipitation, requiring significantly fewer cells or tissue per individual assay. It also has an inherently lower background than ChIP-seq, so each sample does not have to be sequenced as deeply, and it is possible to include more samples in your next-generation sequencing (NGS) flow cell lane. When compared to ChIP-seq, CUT&Tag delivers:

  • Time-savings: ~50% faster chromatin profiling
  • Sample-savings: ~40-fold less sample required
  • Cost-savings: ~2M sequencing reads required per sample

Watch this webinar to learn:

  • How CUT&Tag works and its benefits
  • What to consider when deciding between CUT&Tag and CUT&RUN for your specific experimental needs
  • How CST ensures the performance of all CUT&Tag kits, reagents, and antibodies so you can generate consistent, reliable, and reproducible results

Resources to Support CUT&Tag: