Render Target: STATIC
Render Timestamp: 2025-01-22T14:03:05.650Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-04-05 20:22:25.502
Product last modified at: 2025-01-01T09:06:32.601Z
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

NBC1/SLC4A4 Antibody #11867

Filter:
  • WB
  • IP
Western Blotting Image 1: NBC1/SLC4A4 Antibody
Western blot analysis of extracts from LNCaP and LN18 cells using NBC1/SLC4A4 Antibody.

To Purchase # 11867

Cat. #

Size

11867S
100 µl

Supporting Data

REACTIVITY H M Mk
SENSITIVITY Endogenous
MW (kDa) 135-145
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NBC1/SLC4A4 Antibody recognizes endogenous levels of total NBC1 protein. This antibody also cross-reacts with 42 and 62 kDa proteins of unknown origin in some cell lines.

Species Reactivity:

Human, Mouse, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NBC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

NBC1/SLC4A4 is an eletrogenic sodium bicarbonate cotransporter. It mediates the coupled movement of Na+ and HCO3- ions across the basolateral membrane of cells. NBC1 regulates secretion and absorption of bicarbonate and modulates intracellular pH (1-3). There are five isoforms of NBC1 produced by alternative splicing including the most studied isoforms, 1 and 2, which have different N termini. The N terminus of isoform 1 contains multiple consensus phosphorylation sites for various kinases, such as PKA, PKC, and CK II, and may play a regulatory role in the biological function of NBC1 (4). Interaction with carbonic anhydrase II, IV, and IX regulates the transporter acitivity of NBC1 (5-7). NBC1 is expressed in various tissues with the exception of isoform 2, which is mainly expressed in kidney proximal tubules (1). Isoform 1 is expressed in the pancreas and corneal endothelium, and at low levels in other tissues including heart, skeletal muscle, liver, and prostate (4). Research studies have shown that mutations in the NBC1 gene are linked to proximal renal tubular acidosis with ocular abnormalities (also known as renal tubular acidosis II).
For Research Use Only. Not For Use In Diagnostic Procedures.
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