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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NT5E/CD73 (D7F9A) Rabbit mAb #13160

Filter:
  • WB
  • IHC
Western Blotting Image 1: NT5E/CD73 (D7F9A) Rabbit mAb
Western blot analysis of extracts from HT-29 and Huh7 cells, and rat eye using NT5E/CD73 (D7F9A) Rabbit mAb.

To Purchase # 13160

Cat. # Size Qty. Price
13160T 20 µl
$158
13160S 100 µl
$339

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 70
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:50 - 1:200
Immunohistochemistry (Paraffin) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #87661.

Protocol

Specificity / Sensitivity

NT5E/CD73 (D7F9A) Rabbit mAb recognizes endogenous levels of total NT5E/CD73 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein.

Background

Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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