NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free) #87661
- WB
- IHC
Supporting Data
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 70 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This product is the carrier free version of product #13160. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total NT5E/CD73 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein.
Background
Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8).
- Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87.
- Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27.
- Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002.
- Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54.
- Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64.
- Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4.
- Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44.
- Zylka, M.J. (2011) Trends Mol Med 17, 188-96.
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