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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:16:05.587
Product last modified at: 2025-04-03T12:15:13.628Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free) #87661

Filter:
  • WB
  • IHC
Western Blotting Image 1: NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from HT-29 and Huh7 cells, and rat eye using NT5E/CD73 (D7F9A) Rabbit mAb. Data were generated using the standard formulation of this product.

To Purchase # 87661

Cat. # Size Qty. Price
87661SF 100 µg
$843

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 70
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

This product is the carrier free version of product #13160. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #13160.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total NT5E/CD73 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein.

Background

Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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