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Render Timestamp: 2025-01-22T10:19:31.146Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-20 06:15:35.536
Product last modified at: 2025-01-01T09:06:45.231Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

IL2-Rα/CD25 (BC96) Mouse mAb (APC Conjugate) #20164

Filter:
  • F
Flow Cytometry Image 1: IL2-Rα/CD25 (BC96) Mouse mAb (APC Conjugate)
Flow cytometric analysis of live human peripheral blood mononuclear cells using IL2-Rα/CD25 (BC96) Mouse mAb (APC Conjugate) (right) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (APC Conjugate) #49083 (left), co-stained with CD4 (RPA-T4) Mouse mAb (FITC Conjugate) #48705.

To Purchase # 20164

Cat. #

Size

20164S
500 µl

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1 kappa
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to allophycocyanin (APC) and tested in-house for direct flow cytometric analysis in human cells.

Product Usage Information

Application Dilution
Flow Cytometry (Live) 1:20

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

IL2-Rα/CD25 (BC96) Mouse mAb (APC Conjugate) recognizes endogenous levels of total IL2-Rα/CD25 protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Human

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).
For Research Use Only. Not For Use In Diagnostic Procedures.
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