Render Target: STATIC
Render Timestamp: 2025-01-22T10:25:26.204Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-20 06:22:29.478
Product last modified at: 2025-01-01T09:06:40.036Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Ly-6G (1A8) Rat mAb (redFluor 710 Conjugate) #23220

Filter:
  • F
Flow Cytometry Image 1: Ly-6G (1A8) Rat mAb (redFluor™ 710 Conjugate)
Flow cytometric analysis of live mouse bone marrow cells using Ly-6G (1A8) Rat mAb (redFluor™ 710 Conjugate) (solid line) compared to concentration-matched Rat Isotype Control (redFluor™ 710 Conjugate) (dashed line).

To Purchase # 23220

Cat. #

Size

23220S
100 µg

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rat IgG2a kappa
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to redFluor™ 710 and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.

Application Dilution
Flow Cytometry (Live) 1:80

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

Ly-6G (1A8) Rat mAb (redFluor™ 710 Conjugate) recognizes endogenous levels of total Ly-6G protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

The Ly-6 complex is a series of genes found on chromosome 15. These genes code for a number of different proteins that can be used as surface markers. The family members vary in their biologic expression and have been shown to be involved in cell signaling and cell adhesion (1). The structure of these proteins includes a motif known as the LU domain that has three loops comprised of disulfide bonds. These bonds are formed by 8 to 10 cysteines that can cause differences in the length of the loops as well as the sequences at each tip (2,4). There are 11 known Ly-6 genes on murine chromosome 15 that code for different proteins. These family members, excluding secreted Ly6/Plaur domain containing 1 coded by the Slurp1 gene, are attached to the cell surface by a GPI anchor near the C terminus. The structure of these proteins may play a role in transmembrane interactions, and downstream signaling cascades (1,2).Ly-6 proteins have been widely used as differentiation markers on hematopoietic cells. The ability to isolate and express specific Ly-6 antibodies through hybridoma technology has allowed researchers to identify unique proteins (1). These proteins are expressed on subsets of immune cells at different stages of development, such as T cells, B cells, monocytes, granulocytes, and macrophages (1-5). 
The 1A8 clone is specific to Ly-6G, which is used as a marker for mouse neutrophils (2,3). It is also expressed by mouse polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) (6). The RB6-8C5 clone recognized both Ly-6G and Ly-6C, also known as Gr-1, and has been found to express on neutrophils, monocytes, dendritic cells, and T cells (2,3).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.