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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

EDC4/Ge-1 Antibody #2548

Filter:
  • WB
  • IF
Western Blotting Image 1: EDC4/Ge-1 Antibody
Western blot analysis of extracts from 293 and Hep G2 cells using EDC4/Ge-1 Antibody.

To Purchase # 2548

Cat. # Size Qty. Price Ships
2548S 100 µl
$306 Jan 22

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 170
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

EDC4/Ge-1 Antibody detects endogenous levels of total EDC4/Ge-1 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EDC4/Ge-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjögren's syndrome patient later diagnosed with primary biliary cirrhosis (1). EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation (2). Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182 (2,3). Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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