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XML generation date: 2024-09-20 06:16:00.905
Product last modified at: 2025-01-01T09:05:31.359Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD45 (HI30) Mouse mAb (PE-Cy7® Conjugate) #28418

Filter:
  • F
Flow Cytometry Image 1: CD45 (HI30) Mouse mAb (PE-Cy7® Conjugate)
Flow cytometric analysis of live human peripheral blood mononuclear cells using CD45 (HI30) Mouse mAb (PE-Cy7® Conjugate) (solid line) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (PE-Cy7® Conjugate) #79339 (dashed line).

To Purchase # 28418

Cat. #

Size

28418S
500 µl

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to PE-Cy7® Conjugate and tested in-house for direct flow cytometric analysis in human cells.

Product Usage Information

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:20
Flow Cytometry (Live) 1:20

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD45 (HI30) Mouse mAb (PE-Cy7® Conjugate) recognizes endogenous levels of total CD45 protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Human

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).
The HI30 antibody is widely used as a marker for human CD45 expression on all leukocytes, including T cells, B cells, monocytes, macrophages, and NK cells.

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cy and CyDye are registered trademarks of GE Healthcare.
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