CCL5/RANTES (E9S2K) Rabbit mAb #36467
- WB
- IHC
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 10 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:150 - 1:600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier-free (BSA and azide free) version of this product see product #57219.
Protocol
Specificity / Sensitivity
CCL5/RANTES (E9S2K) Rabbit mAb recognizes endogenous levels of total CCL5/RANTES protein. This antibody recognizes human CCL5/RANTES protein and is also reactive with mouse CCL5/RANTES; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Non-specific staining was observed in human pancreatic islets by immunohistochemistry.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with human CCL5/RANTES recombinant protein.
Background
RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the C-C or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8+ T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts, and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types, including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G protein-coupled receptors (GPCRs), including CCR1, CCR3, CCR4, and CCR5.
- Schall, T.J. (1991) Cytokine 3, 165-83.
- Schall, T.J. et al. (1988) J Immunol 141, 1018-25.
- Kameyoshi, Y. et al. (1992) J Exp Med 176, 587-92.
- Rathanaswami, P. et al. (1993) J Biol Chem 268, 5834-9.
- Mattei, S. et al. (1994) Int J Cancer 56, 853-7.
- Stellato, C. et al. (1995) J Immunol 155, 410-8.
- Schall, T.J. et al. (1990) Nature 347, 669-71.
- Kuna, P. et al. (1992) J Immunol 149, 636-42.
- Mattoli, S. et al. (1995) Biochem Biophys Res Commun 209, 316-21.
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