Render Target: STATIC
Render Timestamp: 2025-01-21T11:05:28.431Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-08-30 10:36:11.333
Product last modified at: 2025-01-01T09:04:14.541Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free) #57219

Filter:
  • WB
  • IHC
Western Blotting Image 1: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using CCL5/RANTES (E9S2K) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.

To Purchase # 57219

Cat. # Size Qty. Price Ships
57219SF 100 µg
$843

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 10
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

This product is the carrier-free version of product #36467. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #36467.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CCL5/RANTES protein. This antibody recognizes human CCL5/RANTES protein and is also reactive with mouse CCL5/RANTES; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Non-specific staining was observed in human pancreatic islets by immunohistochemistry.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with human CCL5/RANTES recombinant protein.

Background

RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the C-C or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8+ T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts, and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types, including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G protein-coupled receptors (GPCRs), including CCR1, CCR3, CCR4, and CCR5.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.