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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

E2-25K/Hip2 Antibody #3847

Filter:
  • WB
Western Blotting Image 1: E2-25K/Hip2 Antibody
Western blot analysis of extracts from various cell types using E2-25K/Hip2 Antibody.

To Purchase # 3847

Cat. # Size Qty. Price Ships
3847S 100 µl
$306

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 25
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

E2-25K/Hip2 Antibody detects endogenous levels of total E2-25K/Hip2 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg11 of human E2-25K/Hip2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).
E2-25K (Hip2) is a member of the E2 protein family that catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin (2). E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, huntingtin, and MHC-heavy chain proteins (3-5). Lys14 of E2-25K can be modified by SUMOylation, with this modification resulting in inhibited E2 activity (6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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