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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate) #58203

Filter:
  • WB
Western Blotting Image 1: Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate)
Western blot analysis of dinitrophenol-conjugated BSA (BSA/DNP, 0.001 ng) using Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate).

To Purchase # 58203

Cat. # Size Qty. Price Ships
58203S 100 µl
$452

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • All-All Species Expected 

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Dinitrophenol (D1D6) Rabbit mAb #14681.

Product Usage Information

Application Dilution
Western Blotting 1:10000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate) recognizes dinitrophenol-conjugated molecules.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with KLH-conjugated dinitrophenol.

Background

The synthetic compound dinitrophenol (DNP) is a small hapten molecule that can be conjugated to proteins for use in immunoassays (1). Antibodies that recognize dinitrophenol will display strong binding affinity to DNP conjugated molecules, including nucleic acids and proteins. These DNP-conjugated compounds can be used in in situ hybridization, western, northern, and Southern blotting (1,2). Dinitrophenol is a commonly used insecticide and has been used as a treatment for weight loss (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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