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Product last modified at: 2025-01-01T09:02:21.799Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dopamine D(2) Receptor/D2R (E1U8K) Rabbit mAb #71241

Filter:
  • IF
Immunofluorescence Image 1: Dopamine D(2) Receptor/D2R (E1U8K) Rabbit mAb
Confocal immunofluorescent analysis of mouse striatum (left, positive) and mouse cerebellum (right, negative) using Dopamine D(2) Receptor/D2R (E1U8K) Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

To Purchase # 71241

Cat. # Size Qty. Price Ships
71241T 20 µl
$153
71241S 100 µl
$339

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Immunofluorescence (Frozen) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Dopamine D(2) Receptor/D2R (E1U8K) Rabbit mAb recognizes endogenous levels of total dopamine D(2) receptor/D2R protein. Signal in the lamina propria of mouse small intestine was observed by Immunofluorescence and is presumed to be non-specific.

Species Reactivity:

Mouse

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Human, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro300 of human dopamine D(2) receptor/D2R protein.

Background

Dopamine is a neurotransmitter that plays important roles in the brain, particularly in dopamine pathways that control the motivational component of reward-motivated behavior. These behavioral outputs are generated by the basal ganglia via its interaction with multiple brain areas that modulate sensorimotor, emotional, and cognitive information (1). The brain’s major dopaminergic input is into the striatum, a region of the basal ganglia composed of GABAergic medium spiny neurons (MSNs). Two major subpopulations of MSN exist in the striatum that are distinguished by the expression of dopamine receptor subtypes, the dopamine D(1) receptor subtype and the dopamine D(2) receptor subtype (D1R and D2R, respectively) (2,3). As a family of proteins, dopamine receptors are a class of G protein-coupled receptors (GPCRs) consisting of 5 subtypes that, upon initiation, drive downstream signaling cascades that modulate neuronal function (1). Dopamine receptors form homo- and hetero-multimers with subunits within their protein family but also with other GPCRs, including Adenosine Receptor A2a, suggesting that dopamine receptor activity might be finely tuned and altered under certain conditions (4). Dopamine receptors have been studied as a therapeutic target for several neuropsychiatric and developmental disorders, as well as neurodegenerative diseases, including Parkinson’s disease (5-8). Dopamine receptors are also expressed outside of the brain and may have diverse functions beyond the central nervous system, including regulating innate and adaptive immunity (9).

Pathways

Explore pathways related to this product.


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