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Product last modified at: 2025-01-16T12:45:38.125Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MCT1/SLC16A1 (E7A2K) Rabbit mAb #76508

Filter:
  • WB
  • IHC
  • IF
  • F
Western Blotting Image 1: MCT1/SLC16A1 (E7A2K) Rabbit mAb
Western blot analysis of extracts from various cell lines using MCT1/SLC16A1 (E7A2K) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of MCT1/SLC16A1 protein in NCI-H3255 cells is consistent with the predicted expression pattern.

To Purchase # 76508

Cat. # Size Qty. Price Ships
76508T 20 µl
$153
76508S 100 µl
$339

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 43
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:50 - 1:200
Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600
Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #49825.

Protocol

Specificity / Sensitivity

MCT1/SLC16A1 (E7A2K) Rabbit mAb recognizes endogenous levels of total MCT1/SLC16A1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly472 of human MCT1/SLC16A1 protein.

Background

MCT1, also known as MOT1, is a multi-pass transmembrane monocarboxylate transporter (MCT) protein, encoded by the gene SLC16A1 (1,2). MCT1 is reported to be expressed in multiple tissues and cell types, including erythrocytes, immune cells, and both cardiac and skeletal myocytes. The primary role of MCT1 is to facilitate proton-coupled transport of monocarboxylates (e.g., lactate, pyruvate, ketone bodies) across the plasma membrane in response to cellular metabolic demands (1). The regulation of monocarboxylate flux by MCT1 has been identified as a possible metabolic biomarker in various diseases, including neurodegenerative diseases, ischemia, and cancer (2-5). Inhibition of MCT1 has thus been proposed as a potential therapeutic treatment of cancer (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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