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Product last modified at: 2025-01-01T09:05:27.415Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD11c (N418) Hamster mAb (PE-Cy7® Conjugate) #77971

Filter:
  • F
Flow Cytometry Image 1: CD11c (N418) Hamster mAb (PE-Cy7® Conjugate)
Flow cytometric analysis of live mouse bone marrow derived dendritic cells using CD11c (N418) Hamster mAb (PE-Cy7® Conjugate) (right), compared to concentration-matched Armenian Hamster Isotype Control (PE-Cy7® Conjugate) (left),

co-stained with MHC Class II (I-A/I-E) (M5/114.15.2) Rat mAb (violetFluor™ 450 Conjugate) #86628.

To Purchase # 77971

Cat. # Size Qty. Price Ships
77971S 100 µg
$217

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Hamster (armenian) IgG
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to PE-Cy7® and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 1.0 μg of antibody per test.

Application Dilution
Flow Cytometry (Live) 1:20

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD11c (N418) Hamster mAb (PE-Cy7® Conjugate) recognizes endogenous levels of total CD11c protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).
The N418 antibody is widely used as a marker for CD11c expression on the cell types mentioned above (8,9).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cy and CyDye are registered trademarks of GE Healthcare.
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