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Product last modified at: 2025-01-01T09:02:08.459Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD45 (30-F11) Rat mAb (violetFluor 450 Conjugate) #83090

Filter:
  • F
Flow Cytometry Image 1: CD45 (30-F11) Rat mAb (violetFluor™ 450 Conjugate)
Flow cytometric analysis of live mouse splenocytes using CD45 (30-F11) Rat mAb (violetFluor™ 450 Conjugate) (solid line) compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (violetFluor™ 450 Conjugate) #52572 (dashed line).

To Purchase # 83090

Cat. # Size Qty. Price
83090S 100 µg
$162

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rat IgG2b kappa
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to violetFluor™ 450 and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.5μg of antibody per test.

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:40
Flow Cytometry (Live) 1:40

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD45 (30-F11) Rat mAb (violetFluor™ 450 Conjugate) recognizes endogenous levels of total mouse CD45 protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).
The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets, and NK cell subsets.
For Research Use Only. Not For Use In Diagnostic Procedures.
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