A2B5 Mouse mAb #8763
- IF
Inquiry Info. # 8763
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Supporting Data
| REACTIVITY | R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgM |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- R-Rat
Product Information
Product Usage Information
It is strongly recommended that these directions be followed for staining of live, unpermeabilized cells when using this antibody. Dilute primary antibody 1:100 in culture medium containing 0.1% sodium azide*. Aspirate cell culture medium, add diluted antibody and incubate cells for 30 minutes at 37ºC. Aspirate antibody solution, rinse gently with pre-warmed PBS, and fix with methanol-free, 4% formaldehyde diluted in PBS for 15 minutes at 37ºC. Rinse cells with PBS 3 times for 5 minutes each. For best results, detect with a fluorochrome-conjugated anti-mouse IgM secondary antibody (per manufacturer's recommendation, diluted in PBS). Prior to imaging, rinse samples in PBS 3 times for 5 minutes each and mount in an anti-fade reagent (ProLong® Gold Antifade Reagent #9071).
* sodium azide is included to prevent capping.
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Limited Uses
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