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Render Timestamp: 2025-01-22T10:19:57.596Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2025-01-15 18:31:08.458
Product last modified at: 2025-01-16T08:00:56.102Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

AQP4x Antibody #60789

Filter:
  • WB
Western Blotting Image 1: AQP4x Antibody
Western blot analysis of extracts from mouse brain and pancreas using AQP4x Antibody (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 as a loading control (lower).

To Purchase # 60789

Cat. #

Size

60789S
100 µl

Supporting Data

REACTIVITY M R
SENSITIVITY Endogenous
MW (kDa) 32
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

AQP4x Antibody recognizes endogenous levels of total mouse AQP4x protein with an extended C-terminal tract.

Species Reactivity:

Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse AQP4x protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Aquaporins (AQPs) are integral membrane proteins that serve as channels to transfer water and small solutes across the membrane. There are 13 isoforms of AQP that are expressed in different types of cells and tissues (1,2). AQP1 is found in blood vessels, kidney, eye, and ear. AQP2 is found in the kidney, and it has been shown that the lack of AQP2 results in diabetes (1,3). AQP4 is present in the brain, where it is enriched in astrocytes (1,2,4). AQP5 is found in the salivary and lacrimal gland, AQP6 in intracellular vesicles in the kidney, AQP7 in adipocytes, AQP8 in kidney, testis, and liver, AQP9 is present in liver and leukocytes, and AQP10-11 in the intestine (1,3,4). AQPs are essential for the function of cells and organs. It has been shown that AQP1 and AQP4 regulate water homeostasis in astrocytes, preventing cerebral edema caused by solute imbalance (5). Several studies have shown the involvement of AQPs in the development of inflammatory processes, including innate and adaptive cell immunity (6,7).

The mouse AQP4x has an extended sequence at the C-terminal (ex) as a result of a codon readthrough during alternative splicing (8). The C-terminal extension in the mouse AQP4x changes the localization to a more perivascular polarization (8). Programmed translational readthrough could occur also in human AQP4x (9).
For Research Use Only. Not For Use In Diagnostic Procedures.
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