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Product last modified at: 2025-01-01T09:00:56.768Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

β-crystallin B1/crybb1 Rabbit Antibody #95666

Filter:
  • WB
Western Blotting Image 1: β-crystallin B1/crybb1 Rabbit Antibody
Western blot analysis of extracts from rat eye, mouse eye, and mouse lung using β-crystallin B1/crybb1 Rabbit Antibody (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

To Purchase # 95666

Cat. # Size Qty. Price Ships
95666S 100 µl
$306

Supporting Data

REACTIVITY M R
SENSITIVITY Endogenous
MW (kDa) 28
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

β-crystallin B1/crybb1 Rabbit Antibody recognizes endogenous levels of total β-crystallin B1 protein.

Species Reactivity:

Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu244 of mouse β-crystallin B1 protein. Antibodies are purified by peptide affinity chromatography.

Background

β-crystallin B1, encoded by crybb1, is a member of the β-crystallins, one of three major crystallin types encoded by the mammalian genome, which also includes alpha- and gamma-crystallins. Crystallins are expressed at very high levels in the retinal lens and form homo/heterodimers via its N-terminal arms (1). Crystallins generally function to maintain the transparency and refractive power of the eye lens. However, they are expressed outside of the lens in other tissues and have alternative non-lens functions (2). For example, Nuc1 mutant rat, in which the βA3/A-crystallin gene is mutated, exhibit vascular remodeling abnormalities via an astrocyte-dependent mechanism (3,4). The precise cellular function of β-crystallins in glia is not well understood, but may involve regulation of endolysosomal acidification, Golgi trafficking, and specific forms of cell death (5,6). Interestingly, crybb1, is highly enriched in microglia outside of the mammalian lens and may serve as a distinct marker for microglia (7). 
For Research Use Only. Not For Use In Diagnostic Procedures.
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