Bcl-11B (D6F1) XP® Rabbit mAb #12120
- WB
- IP
- IF
- ChIP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120-130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Frozen) | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #16148.
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
- Avram, D. et al. (2000) J Biol Chem 275, 10315-22.
- Liu, P. et al. (2010) Immunol Rev 238, 138-49.
- Kominami, R. (2012) Proc Jpn Acad Ser B Phys Biol Sci 88, 72-87.
- Wakabayashi, Y. et al. (2003) Biochem Biophys Res Commun 301, 598-603.
- Cismasiu, V.B. et al. (2005) Oncogene 24, 6753-64.
- Topark-Ngarm, A. et al. (2006) J Biol Chem 281, 32272-83.
- Marban, C. et al. (2007) EMBO J 26, 412-23.
- Gutierrez, A. et al. (2011) Blood 118, 4169-73.
- Obata, M. et al. (2012) Cell Signal 24, 1047-52.
Limited Uses
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