Render Target: STATIC
Render Timestamp: 2024-11-19T11:31:43.123Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:10.091
Product last modified at: 2024-11-18T21:15:08.289Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CCL5/RANTES (E9S2K) Rabbit mAb #36467

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 10
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:150 - 1:600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier-free (BSA and azide free) version of this product see product #57219.

    Protocol

    Specificity / Sensitivity

    CCL5/RANTES (E9S2K) Rabbit mAb recognizes endogenous levels of total CCL5/RANTES protein. This antibody recognizes human CCL5/RANTES protein and is also reactive with mouse CCL5/RANTES; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Non-specific staining was observed in human pancreatic islets by immunohistochemistry.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with human CCL5/RANTES recombinant protein.

    Background

    RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the C-C or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8+ T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts, and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types, including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G protein-coupled receptors (GPCRs), including CCR1, CCR3, CCR4, and CCR5.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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