L-asparaginase/ASRGL1 Antibody #65552
- WB
Supporting Data
| REACTIVITY | H R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 41 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
L-asparaginase/ASRGL1 Antibody recognizes endogenous levels of total L-asparaginase/ASRGL1 protein.
Species Reactivity:
Human, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human L-asparaginase/ASRGL1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
L-asparaginase (ASRGL1) catalyzes the conversion of L-asparagine to L-aspartate. Research studies have shown that intracellular asparagine can suppress apoptosis in a large number of human tumors (1). In addition, acute lymphocytic leukemia cells frequently depend upon serum asparagine for their viability, as they lack asparagine synthetase (ASNS). Deprivation of asparagine by L-asparaginase has therefore been developed as a therapeutic treatment for acute lymphocytic leukemia (2-3). In KRAS mutant non-small cell lung carcinoma (NSCLC) cells, PI3K/Akt signaling was shown to be required for ASNS expression, suggesting combinatorial Akt inhibition and L-asparaginase treatment as a therapeutic strategy for NSCLC (3). Research studies on a breast cancer model have furthermore shown that restriction of asparagine can suppress cancer metastasis (4).
Limited Uses
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